2012年9月21日，中科院南京土壤研究所施卫明、张建华、许卫锋利用NMT在Journal of Experimental Botany上发表了标题为PIN2 is required for the adaptation of Arabidopsis roots to alkaline stress by modulating proton secretion的研究成果。

 

• 期刊：Journal of Experimental Botany
• 主题：PIN2调节碱胁迫下质子分泌
• 标题：PIN2 is required for the adaptation of Arabidopsis roots to alkaline stress by modulating proton secretion
• 影响因子：4.818
• 检测指标：H+流速
• 检测部位：拟南芥根部（从根冠交界处开始50,100,150,200,250,300,400,520,600,700,750,850,900,1000,1100,1200和1500μm）
• H+流实验处理方法：15日龄拟南芥，碱性胁迫（pH 8.0）处理12小时，24小时或48小时
• H+流实验测试液成份：文献无
• 推荐测试液：0.1mM CaCl2，0.3mM MES，pH6.0
• 作者：中科院南京土壤研究所施卫明、张建华、许卫锋

 

英文摘要

Soil alkalinity is a widespread environmental problem that limits agricultural productivity. The hypothesis that an auxin-regulated proton secretion by plasma membrane H+-ATPase plays an important role in root adaption to alkaline stress was studied. It was found that alkaline stress increased auxin transport and PIN2 (an auxin efflux transporter) abundance in the root tip of wild-type Arabidopsis plants (WT).

Compared with WT roots, the pin2 mutant roots exhibited much reduced plasma membrane H+-ATPase activity, root elongation, auxin transport, and proton secretion under alkaline stress. More importantly, roots of the pks5 mutant (PKS5, a protein kinase) lacking PIN2 (a pks5/pin2 double mutant) lost the previous higher proton-secretion capacity and higher elongation rate of primary roots under alkaline stress.

By using Arabidopsis natural accessions with a high proton-secretion capacity, it was found that their PIN2 transcription abundance is positively related to the elongation rate of the primary root and proton-secretion capacity under alkaline stress.

Taken together, our results confirm that PIN2 is involved in the PKS5-mediated signalling cascade under alkaline-stress and suggest that PIN2 is required for the adaptation of roots to alkaline stress by modulating proton secretion in the root tip to maintain primary root elongation.

 

中文摘要（谷歌机翻）

土壤碱度是一个普遍的环境问题，限制了农业生产力。研究了质膜H + -ATPase对生长素调节的质子分泌在根系适应碱胁迫中起重要作用的假设。发现碱胁迫增加了野生型拟南芥植物（WT）根尖中的生长素转运和PIN2（生长素外排转运蛋白）丰度。

与WT根相比，pin2突变体根在碱胁迫下表现出大大降低的质膜H + -ATPase活性，根伸长，生长素转运和质子分泌。更重要的是，缺乏PIN2的pks5突变体（PKS5，蛋白激酶）的根（pks5 / pin2双突变体）在碱性胁迫下丧失了先前较高的质子分泌能力和较高的初生根伸长率。

通过使用具有高质子分泌能力的拟南芥天然种质，发现它们的PIN2转录丰度与初级根的伸长率和碱性胁迫下的质子分泌能力正相关。

总之，我们的结果证实PIN2在碱性胁迫下参与PKS5介导的信号级联反应，并且表明PIN2是通过调节根尖中的质子分泌以维持初级根伸长而使根适应碱性胁迫所必需的。

 

文章链接：

https://academic.oup.com/jxb/article/63/17/6105/563009

 

标签: H+, PIN2, 拟南芥, 碱胁迫, 质子分泌