• 研究使用平台：NMT生物膜创新科研平台
• 期刊：Science of the Total Environment
• 主题：抑制钾信号影响地杆菌生物膜的形成
• 标题：Potassium channel blocker inhibits the formation and electroactivity of Geobacter biofilm
• 影响因子：5.589
• 检测指标：K+流速
• 检测样品：地杆菌
• K+流实验处理方法：

地杆菌分别在0mM、5mM、10mM的TEA中处理

• K+流实验测试液成份：
  0.1mM KCl、1mM glucose
• 作者：福建农林大学陈姗姗、靖宪月

中文摘要（谷歌机翻）

生物膜中的细菌能够利用钾离子通道介导的电信号来实现细胞间的通信。然而，目前尚不清楚当被强电场包围时，这些信号是否在Geobacter sp。中起作用。

这项研究使用了钾通道阻滞剂（四乙铵，TEA），该阻滞剂干扰K+的释放，但不干扰细菌的生长，以证明钾离子通道介导的电信号传导影响了Geobacter sulfreducens的形成和电活性。结果表明，5 mM TEA减慢了地球还原硫细菌生物膜的形成，电流密度比对照组低约50％。

电化学分析表明，加入TEA的生物膜的电活性较差。特别是，具有TEA的微米级生物膜表现出较少的高电流峰，并且参与还原性减少硫杆菌的生物膜中电子转移的最外层基团的物种与对照组不同。

这项工作提供了初步的证据来揭示钾通道在还原性土壤杆菌中的电活性生物膜的作用。

Mean net K+ fluxmeasured from the biofilms in response to treatment with 5 mMand 10mM TEA

英文摘要

Bacteria in biofilms are able to utilize potassium ion channel mediated electrical signaling to achieve cell–cell communication. However, it remains unclear whether these signals play a role in Geobacter sp.when surrounded by an intense electric field.

This study used a potassium channel blocker (tetraethylammonium, TEA) that interfered with the release of K+ but not bacterial growth to demonstrate that potassium ion channel-mediated electrical signaling affected the formation and electroactivity of Geobacter sulfurreducens. The results showed that 5 mM TEA slowed the formation of Geobacter sulfurreducens biofilm, and the current density was ~50% lower than in the control.

The electrochemical analyses showed that the electroactivity of the biofilms with TEA addition was inferior. In particular, the micrometer- scale biofilm with TEA exhibited fewer high current peaks, and the species of outermost groups that participated in the electron transfer in Geobacter sulfurreducens biofilms was different from the control.

This work provides initial evidence to reveal the role of potassium channels in Geobacter sulfurreducens electroactive biofilms.

文章链接：

https://doi.org/10.1016/j.envpol.2020.114103